Journal:

Article Title: Pattern of pro-inflammatory cytokine induction in RAW264.7 mouse macrophages is identical for virulent and attenuated Borrelia burgdorferi 1

doi:

Figure Lengend Snippet: Cytokine and related genes significantly induced or repressed in RAW264.7 gamma NO(−) cells exposed to either B. burgdorferi isolates BL206 or B356 for 16 hours.

Article Snippet: Culture of RAW264.7 gamma NO(−) cells Murine macrophage-like cell line RAW264.7 gamma NO(−) was obtained from the ATCC (CRL-2278, Manassas, VA).

Techniques: Activation Assay

Journal:

Article Title: Pattern of pro-inflammatory cytokine induction in RAW264.7 mouse macrophages is identical for virulent and attenuated Borrelia burgdorferi 1

doi:

Figure Lengend Snippet: B. burgdorferi isolate-specific regulation of cytokine and related genes in the murine macrophage-derived cell line RAW264.7 gamma NO(−) after 16 hours co-culture.

Article Snippet: Culture of RAW264.7 gamma NO(−) cells Murine macrophage-like cell line RAW264.7 gamma NO(−) was obtained from the ATCC (CRL-2278, Manassas, VA).

Techniques:

Journal: Gut Microbes

Article Title: Lachnospiraceae bacterium alleviates alcohol-associated liver disease by enhancing N-acetyl-glutamic acid levels and inhibiting ferroptosis through the KEAP1-NRF2 pathway

doi: 10.1080/19490976.2025.2517821

Figure Lengend Snippet: The abundance of Lachnospiraceae bacterium is significantly reduced in ALD patients and EtOH-fed mice and negatively correlates with liver injury in ALD patients. (a) The comparison of the relative abundance of gut bacteria between HC and ALD groups at the family level (statistical differences were analyzed using two-sided Welch’s t-test of STAMP). (b) The comparison of the relative abundance of gut bacteria between pair-fed and EtOH groups at the family level (statistical differences were analyzed using two-sided Welch’s t-test of STAMP). (c) Indicator analysis between HC and ALD groups. The bubble plot visualizes biomarkers; indval represents the ratio of specificity to occupancy. (d) Relative abundance of Lachnospiraceae bacterium between HC and ALD groups. (e) Indicator analysis between pair-fed and EtOH groups. (f) Relative abundance of Lachnospiraceae bacterium between pair-fed and EtOH groups. (g) Correlation analysis of differential species with serum ALT, AST, and GGT levels. n = 20 per group. HC: healthy controls; ALD: alcohol-associated liver disease patients. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Lachnospiraceae bacterium (ATCC BAA-2278) was cultured under anaerobic conditions at 37°C.

Techniques: Comparison, Bacteria

Journal: Gut Microbes

Article Title: Lachnospiraceae bacterium alleviates alcohol-associated liver disease by enhancing N-acetyl-glutamic acid levels and inhibiting ferroptosis through the KEAP1-NRF2 pathway

doi: 10.1080/19490976.2025.2517821

Figure Lengend Snippet: Lachnospiraceae bacterium attenuates alcohol-associated steatohepatitis in mice. (a) Schematic of experimental design (yellow arrows indicate gavage with PBS or Lachnospiraceae bacterium every other day). (b) Serum ALT and AST levels. (c) Liver TG levels. (d) Representative H&E and Oil red O staining of liver tissue (scale bar: 100 μm). (e) Proportion of neutrophils analyzed by flow cytometry, with corresponding statistical graphs. (f) Hepatic mRNA expression of inflammatory cytokines ( IL-1β, TNF-α, IL-6 ). (g) Relative expression of oxidative stress markers (MDA, SOD, GSH) in liver tissue. n = 6–8 per group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Lachnospiraceae bacterium (ATCC BAA-2278) was cultured under anaerobic conditions at 37°C.

Techniques: Staining, Flow Cytometry, Expressing

Journal: Gut Microbes

Article Title: Lachnospiraceae bacterium alleviates alcohol-associated liver disease by enhancing N-acetyl-glutamic acid levels and inhibiting ferroptosis through the KEAP1-NRF2 pathway

doi: 10.1080/19490976.2025.2517821

Figure Lengend Snippet: Lachnospiraceae bacterium modulates gut microbiota composition and increases metabolite N-Acetyl-glutamic acid. (a) Serum LPS levels. (b) Serum FITC-dextran levels. (c) Protein levels of ZO-1, occludin, and β-actin in ileum tissue with statistical analysis (Pair-fed+PBS vs Pair-fed+Lb vs EtOH+PBS vs EtOH+Lb). (d) PCoA plot of gut microbiota composition (based on Bray-Curtis distances). (e) Heatmap of species-level bacterial composition between EtOH+PBS and EtOH+Lb groups. (f) Histogram showing the relative abundance of Lachnospiraceae bacterium between EtOH+PBS and EtOH+Lb groups. (g) VIP plot of differential metabolites distinguishing EtOH+PBS and EtOH+Lb groups. (h) Histogram of NAG levels between EtOH+PBS and EtOH+Lb groups. (i) Heatmap showing Spearman’s correlation between gut microbiota and metabolites. (j) Portal vein serum NAG levels. n = 6–8 per group. * p < 0.05, ** p < 0.01, *** p < 0.01. For (C): * p < 0.05, ** p < 0.01 and *** p < 0.001 vs Pair-fed+PBS, # p < 0.05, ## p < 0.01, ### p < 0.001 vs EtOH+PBS.

Article Snippet: Lachnospiraceae bacterium (ATCC BAA-2278) was cultured under anaerobic conditions at 37°C.

Techniques:

Journal: Gut Microbes

Article Title: Lachnospiraceae bacterium alleviates alcohol-associated liver disease by enhancing N-acetyl-glutamic acid levels and inhibiting ferroptosis through the KEAP1-NRF2 pathway

doi: 10.1080/19490976.2025.2517821

Figure Lengend Snippet: Lachnospiraceae bacterium and NAG inhibit ferroptosis in alcohol-associated steatohepatitis. (a) Histogram of differential proteins (VIP > 1, p < 0.05) between EtOH and EtOH+Lb groups. (b) Heatmap of differential proteins (VIP > 1, p < 0.05) between EtOH and EtOH+Lb groups. (c) KEGG-enriched pathway analysis. (d) PPI network of ferroptosis-related proteins. (e) Protein levels of NRF2, KEAP1, HO-1, TFRC, FTL1, and β-actin in liver tissue with statistical analysis (Pair-fed+PBS vs Pair-fed+Lb vs EtOH+PBS vs EtOH+Lb). (f) Protein levels of NRF2, KEAP1, HO-1, TFRC, FTL1, and β-actin in liver tissue with statistical analysis (Pair-fed vs Pair-fed+NAG vs EtOH vs EtOH+NAG). (g) Hepatic ferrous ion concentrations. n = 6–8 per group. * p < 0.05, ** p < 0.01, *** p < 0.001. For (E, F): * p < 0.05, ** p < 0.01 and *** p < 0.001 vs Pair-fed+PBS/Pair-fed, # p < 0.05, ## p < 0.01, ### p < 0.001 vs EtOH+PBS/EtOH. Animal model: n = 6–8 per group; Cell model: n = 3 per group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Lachnospiraceae bacterium (ATCC BAA-2278) was cultured under anaerobic conditions at 37°C.

Techniques: Animal Model